Hot Sale 2019-Novel Coronavirus Real-time PCR Test Kits 48T/kit PCR

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This product is intended for the detection of 2019-Novel Coronavirus (2019-nCoV).

The detection result of this product is only for clinical reference, and it should not be used as the only evidence for clinical diagnosis and treatment.

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Hot Sale 2019-Novel Coronavirus Real-time PCR Test Kits 48T/kit PCR

[Principle of Detection]

This product is a multiplex fluorescent probe-based Taqman RT-qPCR assay system. The Taqman fluorescent probe is a specific oligonucleotide based on a reporter-quencher mechanism. For each probe, the 5’-end is labeled with a fluorophore, while the 3’-end was labeled with a quencher. When the probe is intact, the fluorescence emitted by the fluorophore is absorbed by the quencher, and no fluorescent signal is detected. However, during amplification of the template, the probe will be degraded due to the 5'-3’ exonuclease activity of Taq DNA polymerase, and the fluorescent reporter and the quencher are cleaved and separated, then a fluorescent signal can be detected. The generation of each molecular amplicon is accompanied by the generation of a fluorescent signal. Real-time monitoring of the entire PCR process can be assessed by monitoring the accumulation of fluorescent signals.

[Storage & Shelf Life]

All reagents should be stored at -20±5°C with protection from light. The reagents are stable for 6 months when stored at the recommended condition. Use foam box and ice seal for transportation, the transportation temperature is controlled between -76 ℃ ~8 ℃ , and the transportation time should not exceed 4 days. Repeated freezing and thawing of the kit should not exceed 3 times.

[Instruments]

Real-time PCR instrument with FAM, TEXAS RED/ROX and HEX/VIC channels, such as ABI7500、ABI Q3、ABI Q6、Roche LightCycler480、Bio-Rad CFX96、 SLAN-96P.

Note: color compensation is required for Roche lightcycler480

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Hot Sale 2019-Novel Coronavirus Real-time PCR Test Kits 48T/kit PCR

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