Free Thyroxine (FT4) Test Kit

This assay is intended for the quantitative determination of Free Thyroxine in human serum and plasma.

【Parameter】

【Technology highlight】

Chemiluminescence Reasonance Energy Transfer (CRET) Principle

【Intended Use】

Immunoassay for the in vitro quantitative determination of free thyroxine in human serum and plasma.

Thyroxine (T4) is the main thyroid hormone secreted into the bloodstream by the thyroid gland. Together with triiodothyronine (T3) it plays a vital role in regulating the body's metabolic rate, influences the cardiovascular system, growth and bone metabolism, and is important for normal development of gonadal functions and nervous system.T4 circulates in the bloodstream as an equilibrium mixture of free and serum bound hormone. Free T4 (fT4) is the unbound and biologically active form, which represents only 0.03 % of the total T4. The remaining T4 is inactive and bound to serum proteins such as thyroxine binding globulin (TBG) (75 %), pre-albumin (15 %), and albumin (10 %).

The determination of free T4 has the advantage of being independent of changes in the concentrations and binding properties of these binding proteins; additional determination of a binding parameter (T-uptake, TBG) is therefore unnecessary. Thus free T4 is a useful tool in clinical routine diagnostics for the assessment of the thyroid status. It should be measured  together with TSH if thyroid disorders are suspected and is also suitable for monitoring thyrosuppressive therapy.

A variety of methods are available for estimating the free thyroid hormone levels. The direct measurement of fT4 and fT3 via equilibrium dialysis or ultrafiltration is mainly used as a reference method for standardizing, the immunological procedures generally used for routine diagnostic purposes. 

【Advantage】

Low transport costs, no cold chain required.

Single packaging to avoid wasting reagents.

Long shelf-life of reagents, breaking through industry challenges.

【Principle】

Competition principle.

The Free Thyroxine (FT4) test kit employs a wash-free and homogeneous strategy based on proximity hybridization-regulated CRET(Chemiluminescence resonance energy transfer).

【Main Components】

10/20/25 Lyophilization sphere reagents

【Storage Conditions and Expiration Date】

Free Thyroxine (FT4) Test Kit can be stably stored for 18 months at 2-8℃ and protected from light. Apply reagent immediately after opening.

【Applicable Instruments】

Free Thyroxine (FT4) Test Kit used for Chemiluminescence Immunoassay Analyzer (HSCL-5000) produced by Nanjing Poclight Biotechnology Co. Ltd.

【Display】

【Application Scenario】